지방이식 FOR DUMMIES

지방이식 for Dummies

지방이식 for Dummies

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Blend gently just before transferring cells to some conical tube made up of 10 mL of pre-warmed wash medium. Observe: Wash the pipette idea and cryovial While using the clean medium a minimum of three periods that can help improve recovery.

If working with Gentle Cell Dissociation Reagent (GCDR), the period of GCDR treatment method will have to be optimized. Incubate organoids with GCDR for eight -ten minutes at 37°C and make use of a P1000 pipette to gently pipette up and down, and visually inspect if organoids split up.

It is vital to quench the dissociation reagent employing FACS buffer or halt medium, by introducing a minimum of precisely the same or double the quantity of the dissociation reagent.

Cross-portion histology followed by hematoxylin and eosin (H&E) staining to assess the thickness of your compact or significant airway epithelium

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Going back for the plate, rinse Just about every perfectly with one mL of FACS buffer and transfer the volume towards the fifteen mL tube. Observe: Continue to keep cell suspension on ice immediately after transfer to the tube until finally willing to run FACS.

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*This is the master resources record for that under protocols—all reagents mentioned in Each and every protocol are summarized.

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